Wuhan Coronavirus: Megathread -

Which country(ies) will fare the worst due to the WuFlu?


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wry wrangler

sometimes the bar eats you
kiwifarms.net
My employer mandated masks recently, even though the current guidance from public health authorities in my province is against mandatory mask wearing. I can't wear a mask or face shield due to problems with a chronic anxiety disorder. Since they refused to allow me to work without a mask, I now have to take off work for the indefinite future, and go on short term disability until the policy is rescinded or my condition improves so I can wear a mask. They enacted the policy for the sake of political expediency; e.g. they wanted or needed to be seen to do something, even if that thing is ineffective or unwarranted. At the same time they're unwilling even to consider that what they're doing might be ill-advised. They're doing this even as the situation in my province is getting better, not worse, as deaths are way down and the province is relaxing its rules on gatherings and business openings.

Our business is not even open to the public, and 'social distancing' is actually practical. I didn't care when they were just reminding us to wash our hands regularly because people should be doing that anyways or keep 2 metres apart because I don't like being close to others anyways. I'd hoped that would be where it'd end, especially as the situation improves.

I'm just ever so tired of all this relentless bullshit. I just wanted to be able to live my life to the maximum extent possible without being bothered by any of this. After all, there's always hysterical bullshit in the news. Now I can't go to work and earn a full paycheque because my employer has succumbed to peak idiocy. I just want to be left alone!
 

Smug Chuckler

Youtube Intellectual
kiwifarms.net

HarblMcDavid

in ur zone, dekin' my harbl
kiwifarms.net

MarvinTheParanoidAndroid

This will all end in tears, I just know it.
True & Honest Fan
kiwifarms.net
Here's a copy of Li-Meng Yan's study.


Archives of this thread page without spoiler tags:


This document is 25 pages so I may have to split it up.
Unusual Features of the SARS-CoV-2 Genome Suggesting Sophisticated Laboratory Modification Rather Than Natural Evolution and Delineation of Its Probable Synthetic Route

Abstract
The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has led to over 910,000 deaths
worldwide and unprecedented decimation of the global economy. Despite its tremendous impact, the
origin of SARS-CoV-2 has remained mysterious and controversial. The natural origin theory, although
widely accepted, lacks substantial support. The alternative theory that the virus may have come from a
research laboratory is, however, strictly censored on peer-reviewed scientific journals. Nonetheless,
SARS-CoV-2 shows biological characteristics that are inconsistent with a naturally occurring, zoonotic
virus. In this report, we describe the genomic, structural, medical, and literature evidence, which, when
considered together, strongly contradicts the natural origin theory. The evidence shows that SARS-CoV-
2 should be a laboratory product created by using bat coronaviruses ZC45 and/or ZXC21 as a template
and/or backbone. Building upon the evidence, we further postulate a synthetic route for SARS-CoV-2,
demonstrating that the laboratory-creation of this coronavirus is convenient and can be accomplished in
approximately six months. Our work emphasizes the need for an independent investigation into the
relevant research laboratories. It also argues for a critical look into certain recently published data, which,
albeit problematic, was used to support and claim a natural origin of SARS-CoV-2. From a public health
perspective, these actions are necessary as knowledge of the origin of SARS-CoV-2 and of how the virus
entered the human population are of pivotal importance in the fundamental control of the COVID-19
pandemic as well as in preventing similar, future pandemics.

Introduction
COVID-19 has caused a world-wide pandemic, the scale and severity of which are unprecedented.
Despite the tremendous efforts taken by the global community, management and control of this pandemic
remains difficult and challenging.

As a coronavirus, SARS-CoV-2 differs significantly from other respiratory and/or zoonotic viruses: it
attacks multiple organs; it is capable of undergoing a long period of asymptomatic infection; it is highly
transmissible and significantly lethal in high-risk populations; it is well-adapted to humans since the very
start of its emergence1; it is highly efficient in binding the human ACE2 receptor (hACE2), the affinity of
which is greater than that associated with the ACE2 of any other potential host2,3.

The origin of SARS-CoV-2 is still the subject of much debate. A widely cited Nature Medicine
publication has claimed that SARS-CoV-2 most likely came from nature4. However, the article and its
central conclusion are now being challenged by scientists from all over the world5-15. In addition, authors
of this Nature Medicine article show signs of conflict of interests16,17, raising further concerns on the
credibility of this publication.

The existing scientific publications supporting a natural origin theory rely heavily on a single piece of
evidence – a previously discovered bat coronavirus named RaTG13, which shares a 96% nucleotide
sequence identity with SARS-CoV-218. However, the existence of RaTG13 in nature and the truthfulness
of its reported sequence are being widely questioned6-9,19-21. It is noteworthy that scientific journals have
clearly censored any dissenting opinions that suggest a non-natural origin of SARS-CoV-28,22. Because of
this censorship, articles questioning either the natural origin of SARS-CoV-2 or the actual existence of
RaTG13, although of high quality scientifically, can only exist as preprints5-9,19-21 or other non-peerreviewed
articles published on various online platforms10-13,23. Nonetheless, analyses of these reports have
repeatedly pointed to severe problems and a probable fraud associated with the reporting of RaTG136,8,9,19-
21. Therefore, the theory that fabricated scientific data has been published to mislead the world’s efforts
in tracing the origin of SARS-CoV-2 has become substantially convincing and is interlocked with the
notion that SARS-CoV-2 is of a non-natural origin.

Consistent with this notion, genomic, structural, and literature evidence also suggest a non-natural
origin of SARS-CoV-2. In addition, abundant literature indicates that gain-of-function research has long
advanced to the stage where viral genomes can be precisely engineered and manipulated to enable the
creation of novel coronaviruses possessing unique properties. In this report, we present such evidence and
the associated analyses. Part 1 of the report describes the genomic and structural features of SARS-CoV-
2, the presence of which could be consistent with the theory that the virus is a product of laboratory
modification beyond what could be afforded by simple serial viral passage. Part 2 of the report describes
a highly probable pathway for the laboratory creation of SARS-CoV-2, key steps of which are supported
by evidence present in the viral genome. Importantly, part 2 should be viewed as a demonstration of how
SARS-CoV-2 could be conveniently created in a laboratory in a short period of time using available
materials and well-documented techniques. This report is produced by a team of experienced scientists
using our combined expertise in virology, molecular biology, structural biology, computational biology,
vaccine development, and medicine.

1. Has SARS-CoV-2 been subjected to in vitro manipulation?
We present three lines of evidence to support our contention that laboratory manipulation is part of the
history of SARS-CoV-2:

i. The genomic sequence of SARS-CoV-2 is suspiciously similar to that of a bat coronavirus
discovered by military laboratories in the Third Military Medical University (Chongqing, China)
and the Research Institute for Medicine of Nanjing Command (Nanjing, China).

ii. The receptor-binding motif (RBM) within the Spike protein of SARS-CoV-2, which determines
the host specificity of the virus, resembles that of SARS-CoV from the 2003 epidemic in a
suspicious manner. Genomic evidence suggests that the RBM has been genetically manipulated.

iii. SARS-CoV-2 contains a unique furin-cleavage site in its Spike protein, which is known to greatly
enhance viral infectivity and cell tropism. Yet, this cleavage site is completely absent in this
particular class of coronaviruses found in nature. In addition, rare codons associated with this
additional sequence suggest the strong possibility that this furin-cleavage site is not the product of
natural evolution and could have been inserted into the SARS-CoV-2 genome artificially by
techniques other than simple serial passage or multi-strain recombination events inside co-infected
tissue cultures or animals.

1.1 Genomic sequence analysis reveals that ZC45, or a closely related bat coronavirus, should be
the backbone used for the creation of SARS-CoV-2

The structure of the ~30,000 nucleotides-long SARS-CoV-2 genome is shown in Figure 1. Searching
the NCBI sequence database reveals that, among all known coronaviruses, there were two related bat
coronaviruses, ZC45 and ZXC21, that share the highest sequence identity with SARS-CoV-2 (each bat
coronavirus is ~89% identical to SARS-CoV-2 on the nucleotide level). Similarity between the genome
of SARS-CoV-2 and those of representative β coronaviruses is depicted in Figure 1. ZXC21, which is 97%
identical to and shares a very similar profile with ZC45, is not shown. Note that the RaTG13 virus is
excluded from this analysis given the strong evidence suggesting that its sequence may have been
fabricated and the virus does not exist in nature2,6-9. (A follow-up report, which summarizes the up-to-date
evidence proving the spurious nature of RaTG13, will be submitted soon)

1600288928320.png

Figure 1. Genomic sequence analysis reveals that bat coronavirus ZC45 is the closest match to SARS-CoV-2.
Top: genomic organization of SARS-CoV-2 (2019-nCoV WIV04). Bottom: similarity plot based on the full-length
genome of 2019-nCoV WIV04. Full-length genomes of SARS-CoV BJ01, bat SARSr-CoV WIV1, bat SARSr-CoV
HKU3-1, bat coronavirus ZC45 were used as reference sequences.

When SARS-CoV-2 and ZC45/ZXC21 are compared on the amino acid level, a high sequence identity
is observed for most of the proteins. The Nucleocapsid protein is 94% identical. The Membrane protein
is 98.6% identical. The S2 portion (2nd half) of the Spike protein is 95% identical. Importantly, the Orf8
protein is 94.2% identical and the E protein is 100% identical.

Orf8 is an accessory protein, the function of which is largely unknown in most coronaviruses, although
recent data suggests that Orf8 of SARS-CoV-2 mediates the evasion of host adaptive immunity by
downregulating MHC-I24. Normally, Orf8 is poorly conserved in coronaviruses25. Sequence blast
indicates that, while the Orf8 proteins of ZC45/ZXC21 share a 94.2% identity with SARS-CoV-2 Orf8,
no other coronaviruses share more than 58% identity with SARS-CoV-2 on this particular protein. The
very high homology here on the normally poorly conserved Orf8 protein is highly unusual.

1600289000774.png

Figure 2. Sequence alignment of the E proteins from different β coronaviruses demonstrates the E protein’s
permissiveness and tendency toward amino acid mutations. A. Mutations have been observed in different strains
of SARS-CoV. GenBank accession numbers: SARS_GD01: AY278489.2, SARS_ExoN1: ACB69908.1,
SARS_TW_GD1: AY451881.1, SARS_Sino1_11: AY485277.1. B. Alignment of E proteins from related bat
coronaviruses indicates its tolerance of mutations at multiple positions. GenBank accession numbers:
Bat_AP040581.1: APO40581.1, RsSHC014: KC881005.1, SC2018: MK211374.1, Bat_NP_828854.1:
NP_828854.1, BtRs-BetaCoV/HuB2013: AIA62312.1, BM48-31/BGR/2008: YP_003858586.1. C. While the early
copies of SARS-CoV-2 share 100% identity on the E protein with ZC45 and ZXC21, sequencing data of SARS-CoV-
2 from April 2020 indicates that mutation has occurred at multiple positions. Accession numbers of viruses: Feb_11:
MN997409, ZC45: MG772933.1, ZXC21: MG772934, Apr_13: MT326139, Apr_15_A: MT263389, Apr_15_B:
MT293206, Apr_17: MT350246. Alignments were done using the MultAlin webserver
(http://multalin.toulouse.inra.fr/multalin/).

The coronavirus E protein is a structural protein, which is embedded in and lines the interior of the
membrane envelope of the virion26. The E protein is tolerant of mutations as evidenced in both SARS
(Figure 2A) and related bat coronaviruses (Figure 2B). This tolerance to amino acid mutations of the E
protein is further evidenced in the current SARS-CoV-2 pandemic. After only a short two-month spread
of the virus since its outbreak in humans, the E proteins in SARS-CoV-2 have already undergone
mutational changes. Sequence data obtained during the month of April reveals that mutations have
occurred at four different locations in different strains (Figure 2C). Consistent with this finding, sequence
blast analysis indicates that, with the exception of SARS-CoV-2, no known coronaviruses share 100%
amino acid sequence identity on the E protein with ZC45/ZXC21 (suspicious coronaviruses published
after the start of the current pandemic are excluded18,27-31). Although 100% identity on the E protein has
been observed between SARS-CoV and certain SARS-related bat coronaviruses, none of those pairs
simultaneously share over 83% identity on the Orf8 protein32. Therefore, the 94.2% identity on the Orf8
protein, 100% identity on the E protein, and the overall genomic/amino acid-level resemblance between
SARS-CoV-2 and ZC45/ZXC21 are highly unusual. Such evidence, when considered together, is
consistent with a hypothesis that the SARS-CoV-2 genome has an origin based on the use of ZC45/ZXC21
as a backbone and/or template for genetic gain-of-function modifications.

Importantly, ZC45 and ZXC21 are bat coronaviruses that were discovered (between July 2015 and
February 2017), isolated, and characterized by military research laboratories in the Third Military Medical
University (Chongqing, China) and the Research Institute for Medicine of Nanjing Command (Nanjing,
China). The data and associated work were published in 201833,34. Clearly, this backbone/template, which
is essential for the creation of SARS-CoV-2, exists in these and other related research laboratories.

What strengthens our contention further is the published RaTG13 virus18, the genomic sequence of
which is reportedly 96% identical to that of SARS-CoV-2. While suggesting a natural origin of SARSCoV-
2, the RaTG13 virus also diverted the attention of both the scientific field and the general public
away from ZC45/ZXC214,18. In fact, a Chinese BSL-3 lab (the Shanghai Public Health Clinical Centre),
which published a Nature article reporting a conflicting close phylogenetic relationship between SARSCoV-
2 and ZC45/ZXC21 rather than with RaTG1335, was quickly shut down for “rectification”36. It is
believed that the researchers of that laboratory were being punished for having disclosed the SARS-CoV-
2—ZC45/ZXC21 connection. On the other hand, substantial evidence has accumulated, pointing to severe
problems associated with the reported sequence of RaTG13 as well as questioning the actual existence of
this bat virus in nature6,7,19-21. A very recent publication also indicated that the receptor-binding domain
(RBD) of the RaTG13’s Spike protein could not bind ACE2 of two different types of horseshoe bats (they
closely relate to the horseshoe bat R. affinis, RaTG13’s alleged natural host)2, implicating the inability of
RaTG13 to infect horseshoe bats. This finding further substantiates the suspicion that the reported
sequence of RaTG13 could have been fabricated as the Spike protein encoded by this sequence does not
seem to carry the claimed function. The fact that a virus has been fabricated to shift the attention away
from ZC45/ZXC21 speaks for an actual role of ZC45/ZXC21 in the creation of SARS-CoV-2.

1.2 The receptor-binding motif of SARS-CoV-2 Spike cannot be born from nature and should have
been created through genetic engineering

The Spike proteins decorate the exterior of the coronavirus particles. They play an important role in
infection as they mediate the interaction with host cell receptors and thereby help determine the host range
and tissue tropism of the virus. The Spike protein is split into two halves (Figure 3). The front or Nterminal
half is named S1, which is fully responsible for binding the host receptor. In both SARS-CoV
and SARS-CoV-2 infections, the host cell receptor is hACE2. Within S1, a segment of around 70 amino
acids makes direct contacts with hACE2 and is correspondingly named the receptor-binding motif (RBM)
(Figure 3C). In SARS-CoV and SARS-CoV-2, the RBM fully determines the interaction with hACE2.
The C-terminal half of the Spike protein is named S2. The main function of S2 includes maintaining trimer
formation and, upon successive protease cleavages at the S1/S2 junction and a downstream S2’ position,
mediating membrane fusion to enable cellular entry of the virus.

1600289270872.png


Figure 3. Structure of the SARS Spike protein and how it binds to the hACE2 receptor. Pictures were generated
based on PDB ID: 6acj37. A) Three spike proteins, each consisting of a S1 half and a S2 half, form a trimer. B) The
S2 halves (shades of blue) are responsible for trimer formation, while the S1 portion (shades of red) is responsible
for binding hACE2 (dark gray). C) Details of the binding between S1 and hACE2. The RBM of S1, which is
important and sufficient for binding, is colored in orange. Residues within the RBM that are important for either
hACE2 interaction or protein folding are shown as sticks (residue numbers follow the SARS Spike sequence).

1600289404605.png

Figure 4. Sequence alignment of the spike proteins from relevant coronaviruses. Viruses being compared include
SARS-CoV-2 (Wuhan-Hu-1: NC_045512, 2019-nCoV_USA-AZ1: MN997409), bat coronaviruses (Bat_CoV_ZC45:
MG772933, Bat_CoV_ZXC21: MG772934), and SARS coronaviruses (SARS_GZ02: AY390556, SARS:
NC_004718.3). Region marked by two orange lines is the receptor-binding motif (RBM), which is important for
interaction with the hACE2 receptor. Essential residues are additionally highlighted by red sticks on top. Region
marked by two green lines is a furin-cleavage site that exists only in SARS-CoV-2 but not in any other lineage B β
coronavirus.

Similar to what is observed for other viral proteins, S2 of SARS-CoV-2 shares a high sequence identity
(95%) with S2 of ZC45/ZXC21. In stark contrast, between SARS-CoV-2 and ZC45/ZXC21, the S1
protein, which dictates which host (human or bat) the virus can infect, is much less conserved with the
amino acid sequence identity being only 69%.

Figure 4 shows the sequence alignment of the Spike proteins from six β coronaviruses. Two are viruses
isolated from the current pandemic (Wuhan-Hu-1, 2019-nCoV_USA-AZ1); two are the suspected
template viruses (Bat_CoV_ZC45, Bat_CoV_ZXC21); two are SARS coronaviruses (SARS_GZ02,
SARS). The RBM is highlighted in between two orange lines. Clearly, despite the high sequence identity
for the overall genomes, the RBM of SARS-CoV-2 differs significantly from those of ZC45 and ZXC21.
Intriguingly, the RBM of SARS-CoV-2 resembles, on a great deal, the RBM of SARS Spike. Although
this is not an exact “copy and paste”, careful examination of the Spike-hACE2 structures37,38 reveals that
all residues essential for either hACE2 binding or protein folding (orange sticks in Figure 3C and what is
highlighted by red short lines in Figure 4) are “kept”. Most of these essential residues are precisely
preserved, including those involved in disulfide bond formation (C467, C474) and electrostatic
interactions (R444, E452, R453, D454), which are pivotal for the structural integrity of the RBM (Figure
3C and 4). The few changes within the group of essential residues are almost exclusively hydrophobic
“substitutions” (I428àL, L443àF, F460àY, L472àF, Y484àQ), which should not affect either
protein folding or the hACE2-interaction. At the same time, majority of the amino acid residues that are
non-essential have “mutated” (Figure 4, RBM residues not labeled with short red lines). Judging from this
sequence analysis alone, we were convinced early on that not only would the SARS-CoV-2 Spike protein
bind hACE2 but also the binding would resemble, precisely, that between the original SARS Spike protein
and hACE223. Recent structural work has confirmed our prediction39.

As elaborated below, the way that SARS-CoV-2 RBM resembles SARS-CoV RBM and the overall
sequence conservation pattern between SARS-CoV-2 and ZC45/ZXC21 are highly unusual. Collectively,
this suggests that portions of the SARS-CoV-2 genome have not been derived from natural quasi-species
viral particle evolution.

If SARS-CoV-2 does indeed come from natural evolution, its RBM could have only been acquired in
one of the two possible routes: 1) an ancient recombination event followed by convergent evolution or 2)
a natural recombination event that occurred fairly recently.

In the first scenario, the ancestor of SARS-CoV-2, a ZC45/ZXC21-like bat coronavirus would have
recombined and “swapped” its RBM with a coronavirus carrying a relatively “complete” RBM (in
reference to SARS). This recombination would result in a novel ZC45/ZXC21-like coronavirus with all
the gaps in its RBM “filled” (Figure 4). Subsequently, the virus would have to adapt extensively in its new
host, where the ACE2 protein is highly homologous to hACE2. Random mutations across the genome
would have to have occurred to eventually shape the RBM to its current form – resembling SARS-CoV
RBM in a highly intelligent manner. However, this convergent evolution process would also result in the
accumulation of a large amount of mutations in other parts of the genome, rendering the overall sequence
identity relatively low. The high sequence identity between SARS-CoV-2 and ZC45/ZXC21 on various
proteins (94-100% identity) do not support this scenario and, therefore, clearly indicates that SARS-CoV-
2 carrying such an RBM cannot come from a ZC45/ZXC21-like bat coronavirus through this convergent
evolutionary route.

the second scenario, the ZC45/ZXC21-like coronavirus would have to have recently recombined
and swapped its RBM with another coronavirus that had successfully adapted to bind an animal ACE2
highly homologous to hACE2. The likelihood of such an event depends, in part, on the general
requirements of natural recombination: 1) that the two different viruses share significant sequence
similarity; 2) that they must co-infect and be present in the same cell of the same animal; 3) that the
recombinant virus would not be cleared by the host or make the host extinct; 4) that the recombinant virus
eventually would have to become stable and transmissible within the host species.

In regard to this recent recombination scenario, the animal reservoir could not be bats because the
ACE2 proteins in bats are not homologous enough to hACE2 and therefore the adaption would not be able
to yield an RBM sequence as seen in SARS-CoV-2. This animal reservoir also could not be humans as
the ZC45/ZXC21-like coronavirus would not be able to infect humans. In addition, there has been no
evidence of any SARS-CoV-2 or SARS-CoV-2-like virus circulating in the human population prior to late
2019. Intriguingly, according to a recent bioinformatics study, SARS-CoV-2 was well-adapted for humans
since the start of the outbreak1.

Only one other possibility of natural evolution remains, which is that the ZC45/ZXC21-like virus and
a coronavirus containing a SARS-like RBM could have recombined in an intermediate host where the
ACE2 protein is homologous to hACE2. Several laboratories have reported that some of the Sunda
pangolins smuggled into China from Malaysia carried coronaviruses, the receptor-binding domain (RBD)
of which is almost identical to that of SARS-CoV-227-29,31. They then went on to suggest that pangolins
are the likely intermediate host for SARS-CoV-227-29,31. However, recent independent reports have found
significant flaws in this data40-42. Furthermore, contrary to these reports27-29,31, no coronaviruses have been
detected in Sunda pangolin samples collected for over a decade in Malaysia and Sabah between 2009 and
201943. A recent study also showed that the RBD, which is shared between SARS-CoV-2 and the reported
pangolin coronaviruses, binds to hACE2 ten times stronger than to the pangolin ACE22, further dismissing
pangolins as the possible intermediate host. Finally, an in silico study, while echoing the notion that
pangolins are not likely an intermediate host, also indicated that none of the animal ACE2 proteins
examined in their study exhibited more favorable binding potential to the SARS-CoV-2 Spike protein than
hACE2 did3. This last study virtually exempted all animals from their suspected roles as an intermediate
host3, which is consistent with the observation that SARS-CoV-2 was well-adapted for humans from the
start of the outbreak1. This is significant because these findings collectively suggest that no intermediate
host seems to exist for SARS-CoV-2, which at the very least diminishes the possibility of a recombinant
event occurring in an intermediate host.

Even if we ignore the above evidence that no proper host exists for the recombination to take place and
instead assume that such a host does exist, it is still highly unlikely that such a recombination event could
occur in nature.

As we have described above, if natural recombination event is responsible for the appearance of SARSCoV-
2, then the ZC45/ZXC21-like virus and a coronavirus containing a SARS-like RBM would have to
recombine in the same cell by swapping the S1/RBM, which is a rare form of recombination. Furthermore,
since SARS has occurred only once in human history, it would be at least equally rare for nature to produce
a virus that resembles SARS in such an intelligent manner – having an RBM that differs from the SARS
RBM only at a few non-essential sites (Figure 4). The possibility that this unique SARS-like coronavirus
would reside in the same cell with the ZC45/ZXC21-like ancestor virus and the two viruses would
recombine in the “RBM-swapping” fashion is extremely low. Importantly, this, and the other
recombination event described below in section 1.3 (even more impossible to occur in nature), would both
have to happen to produce a Spike as seen in SARS-CoV-2.

While the above evidence and analyses together appear to disapprove a natural origin of SARS-CoV-
2’s RBM, abundant literature shows that gain-of-function research, where the Spike protein of a
coronavirus was specifically engineered, has repeatedly led to the successful generation of humaninfecting
coronaviruses from coronaviruses of non-human origin44-47.

Record also shows that research laboratories, for example, the Wuhan Institute of Virology (WIV),
have successfully carried out such studies working with US researchers45 and also working alone47. In
addition, the WIV has engaged in decades-long coronavirus surveillance studies and therefore owns the
world’s largest collection of coronaviruses. Evidently, the technical barrier is non-existent for the WIV
and other related laboratories to carry out and succeed in such Spike/RBM engineering and gain-offunction
research.
1600290016023.png

Figure 5. Two restriction sites are present at either end of the RBM of SARS-CoV-2, providing convenience for
replacing the RBM within the spike gene. A. Nucleotide sequence of the RBM of SARS-CoV-2 (Wuhan-Hu-1). An
EcoRI site is found at the 5’-end of the RBM and a BstEII site at the 3’-end. B. Although these two restriction sites
do not exist in the original spike gene of ZC45, they can be conveniently introduced given that the sequence
discrepancy is small (2 nucleotides) in either case. C. Amino acid sequence alignment with the RBM region
highlighted (color and underscore). The RBM highlighted in orange (top) is what is defined by the EcoRI and BstEII
sites in the SARS-CoV-2 (Wuhan-Hu-1) spike. The RBM highlighted in magenta (middle) is the region swapped by
Dr. Fang Li and colleagues into a SARS Spike backbone39. The RBM highlighted in blue (bottom) is from the Spike
protein (RBM: 424-494) of SARS-BJ01 (AY278488.2), which was swapped by the Shi lab into the Spike proteins of
different bat coronaviruses replacing the corresponding segments47.

Strikingly, consistent with the RBM engineering theory, we have identified two unique restriction sites,
EcoRI and BstEII, at either end of the RBM of the SARS-CoV-2 genome, respectively (Figure 5A). These
two sites, which are popular choices of everyday molecular cloning, do not exist in the rest of this spike
gene. This particular setting makes it extremely convenient to swap the RBM within spike, providing a
quick way to test different RBMs and the corresponding Spike proteins.

Such EcoRI and BstEII sites do not exist in the spike genes of other β coronaviruses, which strongly
indicates that they were unnatural and were specifically introduced into this spike gene of SARS-CoV-2
for the convenience of manipulating the critical RBM. Although ZC45 spike also does not have these two
sites (Figure 5B), they can be introduced very easily as described in part 2 of this report.

It is noteworthy that introduction of the EcoRI site here would change the corresponding amino acids
from -WNT- to -WNS- (Figure 5AB). As far as we know, all SARS and SARS-like bat coronaviruses
exclusively carry a T (threonine) residue at this location. SARS-CoV-2 is the only exception in that this T
has mutated to an S (serine), save the suspicious RaTG13 and pangolin coronaviruses published after the
outbreak48.

Once the restriction sites were successfully introduced, the RBM segment could be swapped
conveniently using routine restriction enzyme digestion and ligation. Although alternative cloning
techniques may leave no trace of genetic manipulation (Gibson assembly as one example), this oldfashioned
approach could be chosen because it offers a great level of convenience in swapping this critical
RBM.

Given that RBM fully dictates hACE2-binding and that the SARS RBM-hACE2 binding was fully
characterized by high-resolution structures (Figure 3)37,38, this RBM-only swap would not be any riskier
than the full Spike swap. In fact, the feasibility of this RBM-swap strategy has been proven39,47. In 2008,
Dr. Zhengli Shi’s group swapped a SARS RBM into the Spike proteins of several SARS-like bat
coronaviruses after introducing a restriction site into a codon-optimized spike gene (Figure 5C)47. They
then validated the binding of the resulted chimeric Spike proteins with hACE2. Furthermore, in a recent
publication, the RBM of SARS-CoV-2 was swapped into the receptor-binding domain (RBD) of SARSCoV,
resulting in a chimeric RBD fully functional in binding hACE2 (Figure 5C)39. Strikingly, in both
cases, the manipulated RBM segments resemble almost exactly the RBM defined by the positions of the
EcoRI and BstEII sites (Figure 5C). Although cloning details are lacking in both publications39,47, it is
conceivable that the actual restriction sites may vary depending on the spike gene receiving the RBM
insertion as well as the convenience in introducing unique restriction site(s) in regions of interest. It is
noteworthy that the corresponding author of this recent publication39, Dr. Fang Li, has been an active
collaborator of Dr. Zhengli Shi since 201049-53. Dr. Li was the first person in the world to have structurally
elucidated the binding between SARS-CoV RBD and hACE238 and has been the leading expert in the
structural understanding of Spike-ACE2 interactions38,39,53-56. The striking finding of EcoRI and BstEII
restriction sites at either end of the SARS-CoV-2 RBM, respectively, and the fact that the same RBM
region has been swapped both by Dr. Shi and by her long-term collaborator, respectively, using restriction
enzyme digestion methods are unlikely a coincidence. Rather, it is the smoking gun proving that the
RBM/Spike of SARS-CoV-2 is a product of genetic manipulation.
 

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MarvinTheParanoidAndroid

This will all end in tears, I just know it.
True & Honest Fan
kiwifarms.net
Mod Edit: This is too long for a single post, so it had to be split into two. I'm sure he apologizes for double posting.
Although it may be convenient to copy the exact sequence of SARS RBM, it would be too clear a sign
of artificial design and manipulation. The more deceiving approach would be to change a few nonessential
residues, while preserving the ones critical for binding. This design could be well-guided by the
high-resolution structures (Figure 3)37,38. This way, when the overall sequence of the RBM would appear
to be more distinct from that of the SARS RBM, the hACE2-binding ability would be well-preserved. We
believe that all of the crucial residues (residues labeled with red sticks in Figure 4, which are the same
residues shown in sticks in Figure 3C) should have been “kept”. As described earlier, while some should
be direct preservation, some should have been switched to residues with similar properties, which would
not disrupt hACE2-binding and may even strengthen the association further. Importantly, changes might
have been made intentionally at non-essential sites, making it less like a “copy and paste” of the SARS
RBM.

1.3 An unusual furin-cleavage site is present in the Spike protein of SARS-CoV-2 and is associated
with the augmented virulence of the virus

Another unique motif in the Spike protein of SARS-CoV-2 is a polybasic furin-cleavage site located at
the S1/S2 junction (Figure 4, segment in between two green lines). Such a site can be recognized and
cleaved by the furin protease. Within the lineage B of β coronaviruses and with the exception of SARSCoV-
2, no viruses contain a furin-cleavage site at the S1/S2 junction (Figure 6)57. In contrast, furincleavage
site at this location has been observed in other groups of coronaviruses57,58. Certain selective
pressure seems to be in place that prevents the lineage B of β coronaviruses from acquiring or maintaining
such a site in nature.

1600290376677.png

Figure 6. Furin-cleavage site found at the S1/S2 junction of Spike is unique to SARS-CoV-2 and absent in other
lineage B β coronaviruses. Figure reproduced from Hoffmann, et al57.

As previously described, during the cell entry process, the Spike protein is first cleaved at the S1/S2
junction. This step, and a subsequent cleavage downstream that exposes the fusion peptide, are both
mediated by host proteases. The presence or absence of these proteases in different cell types greatly
affects the cell tropism and presumably the pathogenicity of the viral infection. Unlike other proteases,
furin protease is widely expressed in many types of cells and is present at multiple cellular and
extracellular locations. Importantly, the introduction of a furin-cleavage site at the S1/S2 junction could
significantly enhance the infectivity of a virus as well as greatly expand its cell tropism — a phenomenon
well-documented in both influenza viruses and other coronaviruses59-65.

If we leave aside the fact that no furin-cleavage site is found in any lineage B β coronavirus in nature
and instead assume that this site in SARS-CoV-2 is a result of natural evolution, then only one
evolutionary pathway is possible, which is that the furin-cleavage site has to be derived from a
homologous recombination event. Specifically, an ancestor β coronavirus containing no furin-cleavage
site would have to recombine with a closely related coronavirus that does contain a furin-cleavage site.

However, two facts disfavor this possibility. First, although some coronaviruses from other groups or
lineages do contain polybasic furin-cleavage sites, none of them contains the exact polybasic sequence
present in SARS-CoV-2 (-PRRAR/SVA-). Second, between SARS-CoV-2 and any coronavirus containing
a legitimate furin-cleavage site, the sequence identity on Spike is no more than 40%66. Such a low level
of sequence identity rules out the possibility of a successful homologous recombination ever occurring
between the ancestors of these viruses. Therefore, the furin-cleavage site within the SARS-CoV-2 Spike
protein is unlikely to be of natural origin and instead should be a result of laboratory modification.

Consistent with this claim, a close examination of the nucleotide sequence of the furin-cleavage site in
SARS-CoV-2 spike has revealed that the two consecutive Arg residues within the inserted sequence (-
PRRA-) are both coded by the rare codon CGG (least used codon for Arg in SARS-CoV-2) (Figure 7)8.
In fact, this CGGCGG arrangement is the only instance found in the SARS-CoV-2 genome where this
rare codon is used in tandem. This observation strongly suggests that this furin-cleavage site should be a
result of genetic engineering. Adding to the suspicion, a FauI restriction site is formulated by the codon
choices here, suggesting the possibility that the restriction fragment length polymorphism, a technique
that a WIV lab is proficient at67, could have been involved. There, the fragmentation pattern resulted from
FauI digestion could be used to monitor the preservation of the furin-cleavage site in Spike as this furincleavage
site is prone to deletions in vitro68,69. Specifically, RT-PCR on the spike gene of the recovered
viruses from cell cultures or laboratory animals could be carried out, the product of which would be
subjected to FauI digestion. Viruses retaining or losing the furin-cleavage site would then yield distinct
patterns, allowing convenient tracking of the virus(es) of interest.

1600290467132.png


Figure 7. Two consecutive Arg residues in the -PRRA- insertion at the S1/S2 junction of SARS-CoV-2 Spike are
both coded by a rare codon, CGG. A FauI restriction site, 5’-(N)6GCGGG-3’, is embedded in the coding sequence
of the “inserted” PRRA segment, which may be used as a marker to monitor the preservation of the introduced
furin-cleavage site.

In addition, although no known coronaviruses contain the exact sequence of -PRRAR/SVA- that is
present in the SARS-CoV-2 Spike protein, a similar -RRAR/AR- sequence has been observed at the S1/S2
junction of the Spike protein in a rodent coronavirus, AcCoV-JC34, which was published by Dr. Zhengli Shi in 201770. It is evident that the legitimacy of -RRAR- as a functional furin-cleavage site has been
known to the WIV experts since 2017.

The evidence collectively suggests that the furin-cleavage site in the SARS-CoV-2 Spike protein may
not have come from nature and could be the result of genetic manipulation. The purpose of this
manipulation could have been to assess any potential enhancement of the infectivity and pathogenicity of
the laboratory-made coronavirus59-64. Indeed, recent studies have confirmed that the furin-cleavage site
does confer significant pathogenic advantages to SARS-CoV-257,68.

1.4 Summary

Evidence presented in this part reveals that certain aspects of the SARS-CoV-2 genome are extremely
difficult to reconcile to being a result of natural evolution. The alternative theory we suggest is that the
virus may have been created by using ZC45/ZXC21 bat coronavirus(es) as the backbone and/or template.
The Spike protein, especially the RBM within it, should have been artificially manipulated, upon which
the virus has acquired the ability to bind hACE2 and infect humans. This is supported by the finding of a
unique restriction enzyme digestion site at either end of the RBM. An unusual furin-cleavage site may
have been introduced and inserted at the S1/S2 junction of the Spike protein, which contributes to the
increased virulence and pathogenicity of the virus. These transformations have then staged the SARSCoV-
2 virus to eventually become a highly-transmissible, onset-hidden, lethal, sequelae-unclear, and
massively disruptive pathogen.

Evidently, the possibility that SARS-CoV-2 could have been created through gain-of-function
manipulations at the WIV is significant and should be investigated thoroughly and independently.

2. Delineation of a synthetic route of SARS-CoV-2

In the second part of this report, we describe a synthetic route of creating SARS-CoV-2 in a laboratory
setting. It is postulated based on substantial literature support as well as genetic evidence present in the
SARS-CoV-2 genome. Although steps presented herein should not be viewed as exactly those taken, we
believe that key processes should not be much different. Importantly, our work here should serve as a
demonstration of how SARS-CoV-2 can be designed and created conveniently in research laboratories by
following proven concepts and using well-established techniques.
Importantly, research labs, both in Hong Kong and in mainland China, are leading the world in
coronavirus research, both in terms of resources and on the research outputs. The latter is evidenced not
only by the large number of publications that they have produced over the past two decades but also by
their milestone achievements in the field: they were the first to identify civets as the intermediate host for
SARS-CoV and isolated the first strain of the virus71; they were the first to uncover that SARS-CoV
originated from bats72,73; they revealed for the first time the antibody-dependent enhancement (ADE) of
SARS-CoV infections74; they have contributed significantly in understanding MERS in all domains
(zoonosis, virology, and clinical studies)75-79; they made several breakthroughs in SARS-CoV-2
research18,35,80. Last but not least, they have the world’s largest collection of coronaviruses (genomic
sequences and live viruses). The knowledge, expertise, and resources are all readily available within the
Hong Kong and mainland research laboratories (they collaborate extensively) to carry out and accomplish
the work described below.

1600290634714.png


2.1 Possible scheme in designing the laboratory-creation of the novel coronavirus

In this sub-section, we outline the possible overall strategy and major considerations that may have
been formulated at the designing stage of the project.

To engineer and create a human-targeting coronavirus, they would have to pick a bat coronavirus as
the template/backbone. This can be conveniently done because many research labs have been actively
collecting bat coronaviruses over the past two decades32,33,70,72,81-85. However, this template virus ideally
should not be one from Dr. Zhengli Shi’s collections, considering that she is widely known to have been
engaged in gain-of-function studies on coronaviruses. Therefore, ZC45 and/or ZXC21, novel bat
coronaviruses discovered and owned by military laboratories33, would be suitable as the
template/backbone. It is also possible that these military laboratories had discovered other closely related
viruses from the same location and kept some unpublished. Therefore, the actual template could be ZC45,
or ZXC21, or a close relative of them. The postulated pathway described below would be the same
regardless of which one of the three was the actual template.

Once they have chosen a template virus, they would first need to engineer, through molecular cloning,
the Spike protein so that it can bind hACE2. The concept and cloning techniques involved in this
manipulation have been well-documented in the literature44-46,84,86. With almost no risk of failing, the
template bat virus could then be converted to a coronavirus that can bind hACE2 and infect humans44-46.

Second, they would use molecular cloning to introduce a furin-cleavage site at the S1/S2 junction of
Spike. This manipulation, based on known knowledge60,61,65, would likely produce a strain of coronavirus
that is a more infectious and pathogenic.

Third, they would produce an ORF1b gene construct. The ORF1b gene encodes the polyprotein Orf1b,
which is processed post-translationally to produce individual viral proteins: RNA-dependent RNA
polymerase (RdRp), helicase, guanidine-N7 methyltransferase, uridylate-specific endoribonuclease, and
2’-O-methyltransferase. All of these proteins are parts of the replication machinery of the virus. Among
them, the RdRp protein is the most crucial one and is highly conserved among coronaviruses. Importantly,
Dr. Zhengli Shi’s laboratory uses a PCR protocol, which amplifies a particular fragment of the RdRp gene,
as their primary method to detect the presence of coronaviruses in raw samples (bat fecal swap, feces, etc).
As a result of this practice, the Shi group has documented the sequence information of this short segment
of RdRp for all coronaviruses that they have successfully detected and/or collected.

Here, the genetic manipulation is less demanding or complicated because Orf1b is conserved and likely
Orf1b from any β coronavirus would be competent enough to do the work. However, we believe that they
would want to introduce a particular Orf1b into the virus for one of the two possible reasons:

1. Since many phylogenetic analyses categorize coronaviruses based on the sequence similarity of
the RdRp gene only18,31,35,83,87, having a different RdRp in the genome therefore could ensure that
SARS-CoV-2 and ZC45/ZXC21 are separated into different groups/sub-lineages in phylogenetic
studies. Choosing an RdRp gene, however, is convenient because the short RdRp segment sequence
has been recorded for all coronaviruses ever collected/detected. Their final choice was the RdRp
sequence from bat coronavirus RaBtCoV/4991, which was discovered in 2013. For
RaBtCoV/4991, the only information ever published was the sequence of its short RdRp segment83,
while neither its full genomic sequence nor virus isolation were ever reported. After amplifying
the RdRp segment (or the whole ORF1b gene) of RaBatCoV/4991, they would have then used it
for subsequent assembly and creation of the genome of SARS-CoV-2. Small changes in the RdRp
sequence could either be introduced at the beginning (through DNA synthesis) or be generated via
passages later on. On a separate track, when they were engaged in the fabrication of the RaTG13
sequence, they could have started with the short RdRp segment of RaBtCoV/4991 without
introducing any changes to its sequence, resulting in a 100% nucleotide sequence identity between
the two viruses on this short RdRp segment83. This RaTG13 virus could then be claimed to have
been discovered back in 2013.

2. The RdRp protein from RaBatCoV/4991 is unique in that it is superior than RdRp from any other
β coronavirus for developing antiviral drugs. RdRp has no homologs in human cells, which makes
this essential viral enzyme a highly desirable target for antiviral development. As an example,
Remedesivir, which is currently undergoing clinical trials, targets RdRp. When creating a novel
and human-targeting virus, they would be interested in developing the antidote as well. Even
though drug discovery like this may not be easily achieved, it is reasonable for them to
intentionally incorporate a RdRp that is more amenable for antiviral drug development.

Fourth, they would use reverse genetics to assemble the gene fragments of spike, ORF1b, and the rest
of the template ZC45 into a cDNA version of the viral genome. They would then carry out in vitro
transcription to obtain the viral RNA genome. Transfection of the RNA genome into cells would allow
the recovery of live and infectious viruses with the desired artificial genome.

Fifth, they would carry out characterization and optimization of the virus strain(s) to improve the fitness,
infectivity, and overall adaptation using serial passage in vivo. One or several viral strains that meet certain
criteria would then be obtained as the final product(s).

2.2 A postulated synthetic route for the creation of SARS-CoV-2
In this sub-section, we describe in more details how each step could be carried out in a laboratory
setting using available materials and routine molecular, cellular, and virologic techniques. A diagram of
this process is shown in Figure 8. We estimate that the whole process could be completed in approximately
6 months.

Step 1: Engineering the RBM of the Spike for hACE2-binding (1.5 months)

The Spike protein of a bat coronavirus is either incapable of or inefficient in binding hACE2 due to the
missing of important residues within its RBM. This can be exemplified by the RBM of the template virus
ZC45 (Figure 4). The first and most critical step in the creation of SARS-CoV-2 is to engineer the Spike
so that it acquires the ability to bind hACE2. As evidenced in the literature, such manipulations have been
carried out repeatedly in research laboratories since 200844, which successfully yielded engineered
coronaviruses with the ability to infect human cells44-46,88,89. Although there are many possible ways that
one can engineer the Spike protein, we believe that what was actually undertaken was that they replaced
the original RBM with a designed and possibly optimized RBM using SARS’ RBM as a guide. As
described in part 1, this theory is supported by our observation that two unique restriction sites, EcoRI and
BstEII, exist at either end of the RBM in the SARS-CoV-2 genome (Figure 5A) and by the fact that such
RBM-swap has been successfully carried out by Dr. Zhengli Shi and by her long-term collaborator and
structure biology expert, Dr. Fang Li39,47.

Although ZC45 spike does not contain these two restriction sites (Figure 5B), they can be introduced
very easily. The original spike gene would be either amplified with RT-PCR or obtained through DNA
synthesis (some changes could be safely introduced to certain variable regions of the sequence) followed
by PCR. The gene would then be cloned into a plasmid using restriction sites other than EcoRI and BstEII.

Once in the plasmid, the spike gene can be modified easily. First, an EcoRI site can be introduced by
converting the highlighted “gaacac” sequence (Figure 5B) to the desired “gaattc” (Figure 5A). The
difference between them are two consecutive nucleotides. Using the commercially available QuikChange
Site-Directed Mutagenesis kit, such a di-nucleotide mutation can be generated in no more than one week.
Subsequently, the BstEII site could be similarly introduced at the other end of the RBM. Specifically, the
“gaatacc” sequence (Figure 5B) would be converted to the desired “ggttacc” (Figure 5A), which would
similarly require a week of time.

Once these restriction sites, which are unique within the spike gene of SARS-CoV-2, were successfully
introduced, different RBM segments could be swapped in conveniently and the resulting Spike protein
subsequently evaluated using established assays.

As described in part 1, the design of an RBM segment could be well-guided by the high-resolution
structures (Figure 3)37,38, yielding a sequence that resembles the SARS RBM in an intelligent manner.
When carrying out the structure-guided design of the RBM, they would have followed the routine and
generated a few (for example a dozen) such RBMs with the hope that some specific variant(s) may be
superior than others in binding hACE2. Once the design was finished, they could have each of the designed
RBM genes commercially synthesized (quick and very affordable) with an EcoRI site at the 5’-end and a
BstEII site at the 3’-end. These novel RBM genes could then be cloned into the spike gene, respectively.
The gene synthesis and subsequent cloning, which could be done in a batch mode for the small library of
designed RBMs, would take approximately one month.

These engineered Spike proteins might then be tested for hACE2-binding using the established
pseudotype virus infection assays45,49,50. The engineered Spike with good to exceptional binding affinities
would be selected. (Although not necessary, directed evolution could be involved here (error-prone PCR
on the RBM gene), coupled with either an in vitro binding assay39,90 or a pseudotype virus infection
assay45,49,50, to obtain an RBM that binds hACE2 with exceptional affinity.)

Given the abundance of literature on Spike engineering44-46,84,86 and the available high-resolution
structures of the Spike-hACE2 complex37,38, the success of this step would be very much guaranteed. By
the end of this step, as desired, a novel spike gene would be obtained, which encodes a novel Spike protein
capable of binding hACE2 with high affinity.

Step 2: Engineering a furin-cleavage site at the S1/S2 junction (0.5 month)

The product from Step 1, a plasmid containing the engineered spike, would be further modified to
include a furin-cleavage site (segment indicated by green lines in Figure 4) at the S1/S2 junction. This
short stretch of gene sequence can be conveniently inserted using several routine cloning techniques,
including QuikChange Site-Directed PCR60, overlap PCR followed by restriction enzyme digestion and
ligation91, or Gibson assembly. None of these techniques would leave any trace in the sequence.
Whichever cloning method was the choice, the inserted gene piece would be included in the primers,
which would be designed, synthesized, and used in the cloning. This step, leading to a further modified
Spike with the furin-cleavage site added at the S1/S2 junction, could be completed in no more than two
weeks.

Step 3: Obtain an ORF1b gene that contains the sequence of the short RdRp segment from RaBtCoV/4991
(1 month, yet can be carried out concurrently with Steps 1 and 2)

Unlike the engineering of Spike, no complicated design is needed here, except that the RdRp gene
segment from RaBtCoV/4991 would need to be included. Gibson assembly could have been used here. In
this technique, several fragments, each adjacent pair sharing 20-40 bp overlap, are combined together in
one simple reaction to assemble a long DNA product. Two or three fragments, each covering a significant
section of the ORF1b gene, would be selected based on known bat coronavirus sequences. One of these
fragments would be the RdRp segment of RaBtCoV/499183. Each fragment would be PCR amplified with
proper overlap regions introduced in the primers. Finally, all purified fragments would be pooled in
equimolar concentrations and added to the Gibson reaction mixture, which, after a short incubation, would
yield the desired ORF1b gene in whole.

Step 4: Produce the designed viral genome using reverse genetics and recover live viruses (0.5 month)

Reverse genetics have been frequently used in assembling whole viral genomes, including coronavirus
genomes67,92-96. The most recent example is the reconstruction of the SARS-CoV-2 genome using the
transformation-assisted recombination in yeast97. Using this method, the Swiss group assembled the entire
viral genome and produced live viruses in just one week97. This efficient technique, which would not leave
any trace of artificial manipulation in the created viral genome, has been available since 201798,99. In
addition to the engineered spike gene (from steps 1 and 2) and the ORF1b gene (from step 3), other
fragments covering the rest of the genome would be obtained either through RT-PCR amplification from
the template virus or through DNA synthesis by following a sequence slightly altered from that of the
template virus. We believe that the latter approach was more likely as it would allow sequence changes
introduced into the variable regions of less conserved proteins, the process of which could be easily guided
by multiple sequence alignments. The amino acid sequences of more conserved functions, such as that of
the E protein, might have been left unchanged. All DNA fragments would then be pooled together and
transformed into yeast, where the cDNA version of the SARS-CoV-2 genome would be assembled via
transformation-assisted recombination. Of course, an alternative method of reverse genetics, one of which
the WIV has successfully used in the past67, could also be employed67,92-96,100. Although some earlier
reverse genetics approaches may leave restriction sites at where different fragments would be joined, these
traces would be hard to detect as the exact site of ligation can be anywhere in the ~30kb genome. Either
way, a cDNA version of the viral genome would be obtained from the reverse genetics experiment.
Subsequently, in vitro transcription using the cDNA as the template would yield the viral RNA genome,
which upon transfection into Vero E6 cells would allow the production of live viruses bearing all of the
designed properties.

Step 5: Optimize the virus for fitness and improve its hACE2-binding affinity in vivo (2.5-3 months)

Virus recovered from step 4 needs to be further adapted undergoing the classic experiment – serial
passage in laboratory animals101. This final step would validate the virus’ fitness and ensure its receptororiented
adaptation toward its intended host, which, according to the analyses above, should be human.
Importantly, the RBM and the furin-cleavage site, which were introduced into the Spike protein separately,
would now be optimized together as one functional unit. Among various available animal models (e.g.
mice, hamsters, ferrets, and monkeys) for coronaviruses, hACE2 transgenic mice (hACE2-mice) should
be the most proper and convenient choice here. This animal model has been established during the study
of SARS-CoV and has been available in the Jackson Laboratory for many years102-104.

The procedure of serial passage is straightforward. Briefly, the selected viral strain from step 4, a
precursor of SARS-CoV-2, would be intranasally inoculated into a group of anaesthetized hACE2-mice.
Around 2-3 days post infection, the virus in lungs would usually amplify to a peak titer. The mice would
then be sacrificed and the lungs homogenized. Usually, the mouse-lung supernatant, which carries the
highest viral load, would be used to extract the candidate virus for the next round of passage. After
approximately 10~15 rounds of passage, the hACE2-binding affinity, the infection efficiency, and the
lethality of the viral strain would be sufficiently enhanced and the viral genome stabilized101. Finally, after
a series of characterization experiments (e.g. viral kinetics assay, antibodies response assay, symptom
observation and pathology examination), the final product, SARS-CoV-2, would be obtained, concluding
the whole creation process. From this point on, this viral pathogen could be amplified (most probably
using Vero E6 cells) and produced routinely.

It is noteworthy that, based on the work done on SARS-CoV, the hACE2-mice, although suitable for
SARS-CoV-2 adaptation, is not a good model to reflect the virus’ transmissibility and associated clinical
symptoms in humans. We believe that those scientists might not have used a proper animal model (such
as the golden Syrian hamster) for testing the transmissibility of SARS-CoV-2 before the outbreak of
COVID-19. If they had done this experiment with a proper animal model, the highly contagious nature of
SARS-CoV-2 would be extremely evident and consequently SARS-CoV-2 would not have been described
as “not causing human-to-human transmission” at the start of the outbreak.

We also speculate that the extensive laboratory-adaptation, which is oriented toward enhanced
transmissibility and lethality, may have driven the virus too far. As a result, SARS-CoV-2 might have lost
the capacity to attenuate on both transmissibility and lethality during its current adaptation in the human
population. This hypothesis is consistent with the lack of apparent attenuation of SARS-CoV-2 so far
despite its great prevalence and with the observation that a recently emerged, predominant variant only
shows improved transmissibility105-108.

Serial passage is a quick and intensive process, where the adaptation of the virus is accelerated.
Although intended to mimic natural evolution, serial passage is much more limited in both time and scale.
As a result, less random mutations would be expected in serial passage than in natural evolution. This is
particularly true for conserved viral proteins, such as the E protein. Critical in viral replication, the E
protein is a determinant of virulence and engineering of it may render SARS-CoV-2 attenuated109-111
Therefore, at the initial assembly stage, these scientists might have decided to keep the amino acid
sequence of the E protein unchanged from that of ZC45/ZXC21. Due to the conserved nature of the E
protein and the limitations of serial passage, no amino acid mutation actually occurred, resulting in a 100%
sequence identity on the E protein between SARS-CoV-2 and ZC45/ZXC21. The same could have
happened to the marks of molecular cloning (restriction sites flanking the RBM). Serial passage, which
should have partially naturalized the SARS-CoV-2 genome, might not have removed all signs of artificial
manipulation.

3. Final remarks

Many questions remain unanswered about the origin of SARS-CoV-2. Prominent virologists have
implicated in a Nature Medicine letter that laboratory escape, while not being entirely ruled out, was
unlikely and that no sign of genetic manipulation is present in the SARS-CoV-2 genome4. However, here
we show that genetic evidence within the spike gene of SARS-CoV-2 genome (restriction sites flanking
the RBM; tandem rare codons used at the inserted furin-cleavage site) does exist and suggests that the
SARS-CoV-2 genome should be a product of genetic manipulation. Furthermore, the proven concepts,
well-established techniques, and knowledge and expertise are all in place for the convenient creation of
this novel coronavirus in a short period of time.

Motives aside, the following facts about SARS-CoV-2 are well-supported:

1. If it was a laboratory product, the most critical element in its creation, the backbone/template virus
(ZC45/ZXC21), is owned by military research laboratories.

2. The genome sequence of SARS-CoV-2 has likely undergone genetic engineering, through which
the virus has gained the ability to target humans with enhanced virulence and infectivity.

3. The characteristics and pathogenic effects of SARS-CoV-2 are unprecedented. The virus is highly
transmissible, onset-hidden, multi-organ targeting, sequelae-unclear, lethal, and associated with
various symptoms and complications.

4. SARS-CoV-2 caused a world-wide pandemic, taking hundreds of thousands of lives and shutting
down the global economy. It has a destructive power like no other.

Judging from the evidence that we and others have gathered, we believe that finding the origin of
SARS-CoV-2 should involve an independent audit of the WIV P4 laboratories and the laboratories of their
close collaborators. Such an investigation should have taken place long ago and should not be delayed any
further.

We also note that in the publication of the chimeric virus SHC015-MA15 in 2015, the attribution of
funding of Zhengli Shi by the NIAID was initially left out. It was reinstated in the publication in 2016 in
a corrigendum, perhaps after the meeting in January 2016 to reinstate NIH funding for gain-of-function
research on viruses. This is an unusual scientific behavior, which needs an explanation for.
 
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MarvinTheParanoidAndroid

This will all end in tears, I just know it.
True & Honest Fan
kiwifarms.net
What is not thoroughly described in this report is the various evidence indicating that several
coronaviruses recently published (RaTG1318, RmYN0230, and several pangolin coronaviruses27-29,31) are
highly suspicious and likely fraudulent. These fabrications would serve no purpose other than to deceive
the scientific community and the general public so that the true identity of SARS-CoV-2 is hidden.
Although exclusion of details of such evidence does not alter the conclusion of the current report, we do
believe that these details would provide additional support for our contention that SARS-CoV-2 is a
laboratory-enhanced virus and a product of gain-of-function research. A follow-up report focusing on such
additional evidence is now being prepared and will be submitted shortly.

Acknowledgements

We would like to thank Daoyu Zhang for sharing with us the findings of mutations in the E proteins in
different sub-groups of β coronaviruses. We also thank all the anonymous scientists and other individuals,
who have contributed in uncovering various facts associated with the origin of SARS-CoV-2.

References:

1. Zhan, S.H., Deverman, B.E. & Chan, Y.A. SARS-CoV-2 is well adapted for humans. What does this
mean for re-emergence? bioRxiv, https://doi.org/10.1101/2020.05.01.073262 (2020).

2. Mou, H. et al. Mutations from bat ACE2 orthologs markedly enhance ACE2-Fc neutralization of SARSCoV-
2. bioRxiv, https://doi.org/10.1101/2020.06.29.178459 (2020).

3. Piplani, S., Singh, P.K., Winkler, D.A. & Petrovsky, N. In silico comparison of spike protein-ACE2
binding affinities across species; significance for the possible origin of the SARS-CoV-2 virus. arXiv,
arXiv:2005.06199 (2020).

4. Andersen, K.G., Rambaut, A., Lipkin, W.I., Holmes, E.C. & Garry, R.F. The proximal origin of SARSCoV-
2. Nat Med 26, 450-452 (2020).

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To the mod overseeing this thread, can you momentarily undo the spoiler tags so I can snag an archive of this page? archive.md doesn't save spoiler-tagged content. Neither does the Wayback machine.
 
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It's HK-47

Meatbag's Bounty of Bodies
Local Moderator
True & Honest Fan
kiwifarms.net
To the mod overseeing this thread, can you momentarily undo the spoiler tags so I can snag an archive of this page? archive.md doesn't save spoiler-tagged content. Neither does the Wayback machine.
Are you not able to edit your own posts to remove them for however long you need?
 

Bender

The life-ender fender Bender
True & Honest Fan
kiwifarms.net
That was my thought, but there are pictures.
He literally just copy/pasted the contents of the PDF he attached to his first post. There was no reason to do that as he'd already attached the PDF.
 
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UntimelyDhelmise

Galar Purge Survivor
kiwifarms.net
Lets face it, something like this happening was just a matter of time. Im actually surprised it took this long to happen. Like we said, the people were far too comfortable, happy and naive to even IMAGINE something even close to this happening, thus they werent prepared, thinking the golden days would last forever.

Now they are stealing our time, freedom and memories. This isnt about health anymore, its about power, its about control. This has been decades in the making. If these good days will come back is entirely up to us, the system has been turned against us (it always was but now its not even being subtle) now we decide what will happen next...

Like V says

View attachment 1600065

We allowed this ultimately.
What would it take then? No seriously, what would it take? Because it looks to me that the public has done nothing but bow their heads lower and lower the longer this power trip has gone on. There's been some protests (actual peaceful ones) here and there and a few lucky states/countries have said "Fuck off" but it's clearly not been nearly enough to push back against this rampant fearmongering tyranny.
 
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